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Addgene

pBAD/HisB (TIRSTD)-sfGFP
(Plasmid #189674)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189674 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD/HisB
  • Backbone manufacturer
    Thermo
  • Total vector size (bp) 4788
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sfGFP
  • Species
    Synthetic
  • Promoter pBAD
  • Tag / Fusion Protein
    • His-T7-EK (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer atgccatagcatttttatcc
  • 3′ sequencing primer gatttaatctgtatcagg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD/HisB (TIRSTD)-sfGFP was a gift from Daniel Daley (Addgene plasmid # 189674 ; http://n2t.net/addgene:189674 ; RRID:Addgene_189674)

  • For your References section:

    Signal amplification of araC pBAD using a standardized translation initiation region. Shilling PJ, Khananisho D, Cumming AJ, Soderstrom B, Daley DO. Synth Biol (Oxf). 2022 Jul 5;7(1):ysac009. doi: 10.1093/synbio/ysac009. eCollection 2022. 10.1093/synbio/ysac009 PubMed 35903559
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