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Addgene

HisSumoOphP
(Plasmid #189672)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189672 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPIC3.5K
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3 ; Additional selection in Pichia Pastorias using G418 to generate expression Pichia strain.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HisSumoOphP
  • Species
    Pichia Pastorias
  • Tag / Fusion Protein
    • HisSumo tagged (N terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    We got the plasmid from our collaborator from Markus Kunzler in ETH.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HisSumoOphP was a gift from Markus Künzler & James Naismith (Addgene plasmid # 189672 ; http://n2t.net/addgene:189672 ; RRID:Addgene_189672)

  • For your References section:

    Molecular basis for the enzymatic macrocyclization of multiply backbone N-methylated peptides. Emmanuel Matabaro, Haigang Song, Fabio Gherlone, Lukas Sonderegger, Andrew Giltrap, Sam Liver, Alvar Gossert, Markus Künzler, James H Naismith. bioRxiv 2022.07.21.500988 10.1101/2022.07.21.500988
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