His8OphMADeltaC6-18mer.
(Plasmid
#189671)
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PurposeHis8 tagged OphMADeltaC6 with TEV cleavable C-terminus to generate truncated 18merTr and 15mer peptides
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 189671 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET24
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHis8OphMA∆C6-TEV18mer
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SpeciesSynthetic
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Tag
/ Fusion Protein
- His8 tagged
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byWe got it from Markus Kunzler lab from ETH.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.07.21.500988v2 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
His8OphMADeltaC6-18mer. was a gift from Markus Künzler & James Naismith (Addgene plasmid # 189671 ; http://n2t.net/addgene:189671 ; RRID:Addgene_189671) -
For your References section:
Molecular basis for the enzymatic macrocyclization of multiply backbone N-methylated peptides. Emmanuel Matabaro, Haigang Song, Fabio Gherlone, Lukas Sonderegger, Andrew Giltrap, Sam Liver, Alvar Gossert, Markus Künzler, James H Naismith. bioRxiv 2022.07.21.500988 10.1101/2022.07.21.500988