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Addgene

His8OphMA-21mer.
(Plasmid #189670)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189670 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET24
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    His8OphMATEV18mer
  • Species
    Synthetic
  • Tag / Fusion Protein
    • His8 tagged (N terminal on backbone)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The plasmid was from our collaborator Markus Kunzler of ETH

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His8OphMA-21mer. was a gift from Markus Künzler & James Naismith (Addgene plasmid # 189670 ; http://n2t.net/addgene:189670 ; RRID:Addgene_189670)

  • For your References section:

    Molecular basis for the enzymatic macrocyclization of multiply backbone N-methylated peptides. Emmanuel Matabaro, Haigang Song, Fabio Gherlone, Lukas Sonderegger, Andrew Giltrap, Sam Liver, Alvar Gossert, Markus Künzler, James H Naismith. bioRxiv 2022.07.21.500988 10.1101/2022.07.21.500988
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