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pcDNA3-hBcan
(Plasmid #18966)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18966 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1+
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Brevican
  • Alt name
    BCAN
  • Alt name
    CSPG7
  • Alt name
    BEHAB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3200
  • GenBank ID
    BC010571
  • Entrez Gene
    BCAN (a.k.a. BEHAB, CSPG7)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Original MGC clone BC010571 purchased from Invitrogen

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Viapiano MS et. al: Novel tumor-specific isoforms of BEHAB/brevican identified in human malignant gliomas. Cancer Res. (2005) 65:6726.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-hBcan was a gift from Mariano Viapiano (Addgene plasmid # 18966 ; http://n2t.net/addgene:18966 ; RRID:Addgene_18966)

  • For your References section:

    The proteoglycan brevican binds to fibronectin after proteolytic cleavage and promotes glioma cell motility. Hu B, Kong LL, Matthews RT, Viapiano MS. J Biol Chem. 2008 Jul 7. ():. 10.1074/jbc.M801433200 PubMed 18611854

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