ODD-Luciferase-pcDNA3
(Plasmid #18965)

• Depositing Lab: William Kaelin
• Publication: Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 103(1):105-10.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5352
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ODD-Firefly Luciferase
• Alt name: HIF1a-ODD
• Species: H. sapiens (human)
• Insert Size (bp): 2047
• Mutation: HIF1a ODD sequence fused in frame to luciferase ORF
• GenBank ID: AAA89082
• Entrez Gene: HIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry