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pLenti_CHyMErA.v2_U6(SpCas9_I-SceI)-(AsCas12a(dual-gRNA)_PacI-PacI)_PGK-puro
(Plasmid #189636)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189636 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLentiCRISPRv2 backbone
  • Total vector size (bp) 9150
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hU6 Cas9-Cas12a-Cas12a gRNA cassette, PGK puro cassette
  • gRNA/shRNA sequence
    non targeting gRNA

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti_CHyMErA.v2_U6(SpCas9_I-SceI)-(AsCas12a(dual-gRNA)_PacI-PacI)_PGK-puro was a gift from Manuel Kaulich (Addgene plasmid # 189636 ; http://n2t.net/addgene:189636 ; RRID:Addgene_189636)

  • For your References section:

    Optimized metrics for orthogonal combinatorial CRISPR screens. Cetin R, Wegner M, Luwisch L, Saud S, Achmedov T, Susser S, Vera-Guapi A, Muller K, Matthess Y, Quandt E, Schaubeck S, Beisel CL, Kaulich M. Sci Rep. 2023 May 6;13(1):7405. doi: 10.1038/s41598-023-34597-8. 10.1038/s41598-023-34597-8 PubMed 37149686
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