pAAV-hSyn-DIO-Gluc-RMA-IRES-EGFP
(Plasmid #189630)

• Purpose: Expresses Gluc-RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-RMA and EGFP for monitoring Cre-expressing neuronal populations.
• Depositing Lab: Jerzy Szablowski
• Publication: Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.1038/s41587-023-02087-x.

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 4813
• Total vector size (bp): 7340
• Vector type: Mammalian Expression, AAV, Cre/Lox, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Gaussia luciferase fused to Fc
• Alt name: Gluc-mouse IgG1 Fc
• Alt name: Gluc-RMA
• Species: M. musculus (mouse), Synthetic; Gaussia princeps
• Promoter: hSyn
• Tags / Fusion Proteins:
  • Gluc (N terminal on insert)
  • IgG1 Fc (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: catagcgtaaaaggagcaaca

Gene/Insert 2

• Gene/Insert name: IRES-EGFP
• Promoter: IRES

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: tcagcgctgcctcagtct

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Gluc is derived from AAV-hSyn-GlucM23-iChloC-EYFP (Addgene #114102).
IgG1 is derived from SI4-Jamc.2 (Addgene #28216).
IRES-EGFP is derived from pAAV.CMV.Luc.IRES.EGFP.SV40 (Addgene #105533).
DIO (double-floxed inverted orientation) backbone is derived from pAAV-hSyn-DIO-EGFP (Addgene #50457).

Please visit https://www.biorxiv.org/content/10.1101/2022.07.17.500352v1 for BioRxiv preprint