HA-EglN1-pcDNA3
(Plasmid #18963)

• Depositing Lab: William Kaelin
• Publication: Ivan et al Proc Natl Acad Sci U S A. 2002 Oct 15. 99(21):13459-64.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Egg laying Nine - 1
• Alt name: PHD2
• Alt name: Prolyl Hydroxylase 2
• Species: H. sapiens (human)
• Insert Size (bp): 1287
• Mutation: wt cDNA
• GenBank ID: NM_022051
• Entrez Gene: EGLN1 (a.k.a. C1orf12, ECYT3, HALAH, HIF-PH2, HIFPH2, HPH-2, HPH2, PHD2, SM20, ZMYND6)
• Tag / Fusion Protein:
  • HA-tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH I (not destroyed)
• 3′ cloning site: EcoR I (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry