pAAV-hSyn-Cluc-RMA
(Plasmid #189624)

• Purpose: Expresses Cluc-RMA under the hSyn promoter. Cluc-RMA for monitoring neuronal transduction.
• Depositing Lab: Jerzy Szablowski
• Publication: Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.1038/s41587-023-02087-x.

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 4569
• Total vector size (bp): 6879
• Vector type: Mammalian Expression, AAV, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cypridina luciferase fused to Fc
• Alt name: Cluc-mouse IgG1 Fc
• Alt name: Cluc-RMA
• Species: M. musculus (mouse), Synthetic; Cypridina noctiluca
• Promoter: hSyn
• Tags / Fusion Proteins:
  • Cluc (N terminal on insert)
  • IgG1 Fc (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: tcagcgctgcctcagtct

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cluc is derived from pCLucIPZ (Addgene #53222).
IgG1 is derived from SI4-Jamc.2 (Addgene #28216).

Please visit https://www.biorxiv.org/content/10.1101/2022.07.17.500352v1 for BioRxiv preprint