pTP-PCR C4
(Plasmid #189621)

• Purpose: Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
• Depositing Lab: Bogumil Karas
• Publication: Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii: biology9110358. doi: 10.3390/biology9110358.

Backbone

• Vector backbone: pAGE3.0
• Backbone manufacturer: Brumwell et al. 2019
• Backbone size w/o insert (bp): 17523
• Total vector size (bp): 57557
• Modifications to backbone: Inserted the T. pseudonana mitochondrial genome with repeat region removed into the backbone of pAGE3.0
• Vector type: Bacterial Expression, Yeast Expression, Synthetic Biology ; Diatom Expression
• Selectable markers: HIS3 ; Nourseothricin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): EPI300 (Epicentre)
• Growth instructions: Optional: Arabinose induction to high plasmid copy number.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Reduced Mitochondrial Genome of Thalassiosira pseudonana
• Alt name: T. pseudonana
• Alt name: Mitochondrial Genome
• Species: T. pseudonana
• Insert Size (bp): 40034
• Mutation: Repeat region removed, and minor additional mutations described in manuscript
• GenBank ID: NC_007405.1

Resource Information

• Supplemental Documents:
  • ptp-pcr-c4.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note this plasmid is larger than what is typically sequenced at Addgene. Discrepancies present in the mitochondrial genome sequence are not of any functional concern for the plasmid.