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Addgene

pTP-PCR C4
(Plasmid #189621)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189621 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAGE3.0
  • Backbone manufacturer
    Brumwell et al. 2019
  • Backbone size w/o insert (bp) 17523
  • Total vector size (bp) 57557
  • Modifications to backbone
    Inserted the T. pseudonana mitochondrial genome with repeat region removed into the backbone of pAGE3.0
  • Vector type
    Bacterial Expression, Yeast Expression, Synthetic Biology ; Diatom Expression
  • Selectable markers
    HIS3 ; Nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300 (Epicentre)
  • Growth instructions
    Optional: Arabinose induction to high plasmid copy number.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Reduced Mitochondrial Genome of Thalassiosira pseudonana
  • Alt name
    T. pseudonana
  • Alt name
    Mitochondrial Genome
  • Species
    T. pseudonana
  • Insert Size (bp)
    40034
  • Mutation
    Repeat region removed, and minor additional mutations described in manuscript
  • GenBank ID
    NC_007405.1

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note this plasmid is larger than what is typically sequenced at Addgene. Discrepancies present in the mitochondrial genome sequence are not of any functional concern for the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTP-PCR C4 was a gift from Bogumil Karas (Addgene plasmid # 189621 ; http://n2t.net/addgene:189621 ; RRID:Addgene_189621)

  • For your References section:

    Cloning of Thalassiosira pseudonana's Mitochondrial Genome in Saccharomyces cerevisiae and Escherichia coli. Cochrane RR, Brumwell SL, Shrestha A, Giguere DJ, Hamadache S, Gloor GB, Edgell DR, Karas BJ. Biology (Basel). 2020 Oct 26;9(11). pii: biology9110358. doi: 10.3390/biology9110358. 10.3390/biology9110358 PubMed 33114477
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