pTP-PCR C3
(Plasmid
#189620)
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PurposeCloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 189620 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAGE3.0
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Backbone manufacturerBrumwell et al. 2019
- Backbone size w/o insert (bp) 17385
- Total vector size (bp) 46588
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Modifications to backboneInserted the T. pseudonana mitochondrial genome with repeat region deleted into the backbone of pAGE3.0
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Vector typeBacterial Expression, Yeast Expression, Synthetic Biology ; Diatom Expression
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Selectable markersHIS3 ; Nourseothricin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)EPI300 (Epicentre)
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Growth instructionsOptional: Arabinose induction to high plasmid copy number.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameReduced Mitochondrial Genome of Thalassiosira pseudonana
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Alt nameT. pseudonana
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Alt nameMitochondrial Genome
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SpeciesT. pseudonana
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Insert Size (bp)29203
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MutationRemoved repeat region, 10-kbp deletion, and minor additional mutations described in manuscript
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GenBank IDNC_007405.1
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note this plasmid is larger than what is typically sequenced at Addgene. Discrepancies present in the mitochondrial genome sequence are not of any functional concern for the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTP-PCR C3 was a gift from Bogumil Karas (Addgene plasmid # 189620 ; http://n2t.net/addgene:189620 ; RRID:Addgene_189620) -
For your References section:
Cloning of Thalassiosira pseudonana's Mitochondrial Genome in Saccharomyces cerevisiae and Escherichia coli. Cochrane RR, Brumwell SL, Shrestha A, Giguere DJ, Hamadache S, Gloor GB, Edgell DR, Karas BJ. Biology (Basel). 2020 Oct 26;9(11). pii: biology9110358. doi: 10.3390/biology9110358. 10.3390/biology9110358 PubMed 33114477