pAAV-Syn1-GRAB-HTR6-cilia
(Plasmid
#189615)
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PurposeExpresses cilia-targeted serotonin sensor based on HTR6 receptor and cpEGFP, driven by SYN promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 189615 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Backbone manufacturerVectorbuilder
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHTR6_GRAB
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Insert Size (bp)3892
- Promoter Syn1
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Made by VectorBuilder
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-Syn1-GRAB-HTR6-cilia was a gift from David Clapham (Addgene plasmid # 189615 ; http://n2t.net/addgene:189615 ; RRID:Addgene_189615) -
For your References section:
A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility. Sheu SH, Upadhyayula S, Dupuy V, Pang S, Deng F, Wan J, Walpita D, Pasolli HA, Houser J, Sanchez-Martinez S, Brauchi SE, Banala S, Freeman M, Xu CS, Kirchhausen T, Hess HF, Lavis L, Li Y, Chaumont-Dubel S, Clapham DE. Cell. 2022 Sep 1;185(18):3390-3407.e18. doi: 10.1016/j.cell.2022.07.026. 10.1016/j.cell.2022.07.026 PubMed 36055200