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Addgene

pB-Tet-On-HTR6-RhoA sensor
(Plasmid #189614)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189614 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Xlone
  • Total vector size (bp) 9408
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HTR6
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_000871.3
  • Entrez Gene
    HTR6 (a.k.a. 5-HT6, 5-HT6R)
  • Promoter Tet3G
  • Tag / Fusion Protein
    • GFP and mScarlet (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB-Tet-On-HTR6-RhoA sensor was a gift from David Clapham (Addgene plasmid # 189614 ; http://n2t.net/addgene:189614 ; RRID:Addgene_189614)

  • For your References section:

    A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility. Sheu SH, Upadhyayula S, Dupuy V, Pang S, Deng F, Wan J, Walpita D, Pasolli HA, Houser J, Sanchez-Martinez S, Brauchi SE, Banala S, Freeman M, Xu CS, Kirchhausen T, Hess HF, Lavis L, Li Y, Chaumont-Dubel S, Clapham DE. Cell. 2022 Sep 1;185(18):3390-3407.e18. doi: 10.1016/j.cell.2022.07.026. 10.1016/j.cell.2022.07.026 PubMed 36055200
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