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Addgene

AAV-GFAP(long)-FLEX-GCaMP7f
(Plasmid #189612)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189612 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Vector Builder
  • Backbone size w/o insert (bp) 3277
  • Total vector size (bp) 7558
  • Modifications to backbone
    Human GFAP promoter FLEX GCaMP7f
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GCaMP7f
  • Species
    Synthetic
  • Insert Size (bp)
    1577
  • Tag / Fusion Protein
    • GCaMP7f (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-GFAP(long)-FLEX-GCaMP7f was a gift from Martin Riccomagno (Addgene plasmid # 189612 ; http://n2t.net/addgene:189612 ; RRID:Addgene_189612)

  • For your References section:

    A genetic tool for the longitudinal study of a subset of post-inflammatory reactive astrocytes. Agnew-Svoboda W, Ubina T, Figueroa Z, Wong YC, Vizcarra EA, Roebini B, Wilson EH, Fiacco TA, Riccomagno MM. Cell Rep Methods. 2022 Aug 22;2(8):100276. doi: 10.1016/j.crmeth.2022.100276. eCollection 2022 Aug 22. 10.1016/j.crmeth.2022.100276 PubMed 36046623
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