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Addgene

pCAG-ATG101
(Plasmid #189590)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189590 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATG101
  • Species
    H. sapiens (human)
  • GenBank ID
    NP_068753.2
  • Entrez Gene
    ATG101 (a.k.a. C12orf44)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (unknown if destroyed)
  • 3′ cloning site XmaI (unknown if destroyed)
  • 5′ sequencing primer 5'-CTTCTTCTTTTTCCTACAGCTCCTGGGC
  • 3′ sequencing primer 5’-gagccagggcattggccacac
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-ATG101 was a gift from James Hurley (Addgene plasmid # 189590 ; http://n2t.net/addgene:189590 ; RRID:Addgene_189590)

  • For your References section:

    ULK complex organization in autophagy by a C-shaped FIP200 N-terminal domain dimer. Shi X, Yokom AL, Wang C, Young LN, Youle RJ, Hurley JH. J Cell Biol. 2020 Jul 6;219(7):e201911047. doi: 10.1083/jcb.201911047. 10.1083/jcb.201911047 PubMed 32516362
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