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Addgene

pcDNA4-rB90
(Plasmid #18959)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18959 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1+
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Brevican C-terminus
  • Alt name
    BCAN
  • Alt name
    BEHAB
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2000
  • Mutation
    Rat brevican C-terminus: Signal peptide aa 1-22 between Hind III and EcoRI, followed by aa 396-883 between Eco RI and NotI (EcoRI site was introduced and created two extra aa)
  • GenBank ID
    NM_001033665
  • Entrez Gene
    Bcan (a.k.a. ALPBRE)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Not I (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Original full-length brevican clone was kindly provided by Dr. Yu Yamaguchi (Burnham Institute, La Jolla, CA)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4-rB90 was a gift from Mariano Viapiano (Addgene plasmid # 18959 ; http://n2t.net/addgene:18959 ; RRID:Addgene_18959)

  • For your References section:

    BEHAB/brevican requires ADAMTS-mediated proteolytic cleavage to promote glioma invasion. Viapiano MS, Hockfield S, Matthews RT. J Neurooncol. 2008 Jul . 88(3):261-72. 10.1007/s11060-008-9575-8 PubMed 18398576
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