pBA1015
(Plasmid
#189589)
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PurposeT7gp1.7 deletion HR vector 250bp HR Arms (T7 phage editing)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 189589 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneBBR1
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameT7gp1.7 Locus 250bp Homology Arms
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SpeciesBacteriophage T7
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MutationEncodes for a full deletion of T7gp1.7
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GenBank IDNC_001604
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBA1015 was a gift from Jennifer Doudna (Addgene plasmid # 189589 ; http://n2t.net/addgene:189589 ; RRID:Addgene_189589) -
For your References section:
Broad-spectrum CRISPR-Cas13a enables efficient phage genome editing. Adler BA, Hessler T, Cress BF, Lahiri A, Mutalik VK, Barrangou R, Banfield J, Doudna JA. Nat Microbiol. 2022 Dec;7(12):1967-1979. doi: 10.1038/s41564-022-01258-x. Epub 2022 Oct 31. 10.1038/s41564-022-01258-x PubMed 36316451