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Addgene

pBA1030
(Plasmid #189582)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189582 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    BBR1
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    EdH4gp0004 Deletion Locus 250bp Homology Arms
  • Species
    Bacteriophage EdH4
  • Mutation
    Encodes for a full deletion of EdH4gp0004
  • GenBank ID
    MK327930.1

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBA1030 was a gift from Jennifer Doudna (Addgene plasmid # 189582 ; http://n2t.net/addgene:189582 ; RRID:Addgene_189582)

  • For your References section:

    Broad-spectrum CRISPR-Cas13a enables efficient phage genome editing. Adler BA, Hessler T, Cress BF, Lahiri A, Mutalik VK, Barrangou R, Banfield J, Doudna JA. Nat Microbiol. 2022 Dec;7(12):1967-1979. doi: 10.1038/s41564-022-01258-x. Epub 2022 Oct 31. 10.1038/s41564-022-01258-x PubMed 36316451
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