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pBbA2C_Ptet::lasI
(Plasmid #189576)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189576 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBbA2C
  • Backbone size w/o insert (bp) 3287
  • Total vector size (bp) 3218
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Acyl-homoserine-lactone synthase
  • Alt name
    luxI
  • Species
    Pseudomonas aeruginosa
  • Insert Size (bp)
    609

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TACCACTCCCTATCAGTGATAGAG
  • 3′ sequencing primer AAGGCCCAGTCTTTCGACTGAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBbA2C_Ptet::lasI was a gift from Daniel Ducat (Addgene plasmid # 189576 ; http://n2t.net/addgene:189576 ; RRID:Addgene_189576)

  • For your References section:

    Developing Cyanobacterial Quorum Sensing Toolkits: Toward Interspecies Coordination in Mixed Autotroph/Heterotroph Communities. Kokarakis EJ, Rillema R, Ducat DC, Sakkos JK. ACS Synth Biol. 2023 Jan 20;12(1):265-276. doi: 10.1021/acssynbio.2c00527. Epub 2022 Dec 27. 10.1021/acssynbio.2c00527 PubMed 36573789
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