pRnc2-gGFP
(Plasmid #189563)

• Purpose: Expresses E. Coli RNase III under control of pBAD promoter with native 5' untranslated region and constitutively expresses gUTR-129-GFP
• Depositing Lab: Marc Ostermeier
• Publication: Weeks et al Mol Biol Evol. 2023 Feb 27:msad047. doi: 10.1093/molbev/msad047.

Backbone

• Vector backbone: pBR322
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 4660
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: RNase III
• Alt name: rnc
• Species: E. coli
• Insert Size (bp): 841
• Mutation: Maintains 5' UTR of rnc
• Promoter: pBAD

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: SacI (not destroyed)
• 5′ sequencing primer: pBad-Forward
• 3′ sequencing primer: pBad-Reverse

Gene/Insert 2

• Gene/Insert name: gUTR-129-GFP
• Alt name: fusion of rnc UTR and gRNA targeting GFP
• Insert Size (bp): 256
• Promoter: J23119

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pRnc2-gGFP.ape
• A portion of this plasmid was derived from a plasmid made by: Addgene - pgRNA (bacterial): Plasmid #44251

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry