pSCM167-Venus-VF-hADAM10(aa220-654)
(Plasmid
#189560)
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PurposeProtein expression ADAM10 in mammalian cells. Contains metalloprotease, disintegrin, and cysteine rich domains--amino acids 220-654
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 189560 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSCM167-Venus (Fu lab)
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Backbone manufacturerCustomized backbone from Fu lab
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametruncated ADAM metallopeptidase domain 10
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Alt nameADAM10; RAK; kuz; AD10; AD18; MADM; CD156c; CDw156; HsT18717
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SpeciesH. sapiens (human)
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Mutationcontains aa 220-654
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Entrez GeneADAM10 (a.k.a. AD10, AD18, CD156c, CDw156, HsT18717, MADM, RAK, kuz)
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Tag
/ Fusion Protein
- VF (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSCM167-Venus-VF-hADAM10(aa220-654) was a gift from Haian Fu (Addgene plasmid # 189560 ; http://n2t.net/addgene:189560 ; RRID:Addgene_189560)