pSCM167.2-Venus-VF-hADAM10(aa1-213)
(Plasmid #189554)

• Purpose: Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213
• Depositing Lab: Haian Fu
• Publication: TREAT-AD plasmids 2022 (unpublished)

Backbone

• Vector backbone: pSCM167.2-Venus (Fu lab)
• Backbone manufacturer: Customized backbone from Fu lab
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: truncated ADAM metallopeptidase domain 10
• Alt name: ADAM10; RAK; kuz; AD10; AD18; MADM; CD156c; CDw156; HsT18717
• Species: H. sapiens (human)
• Mutation: contains aa 1-213
• Entrez Gene: ADAM10 (a.k.a. AD10, AD18, CD156c, CDw156, HsT18717, MADM, RAK, kuz)
• Tag / Fusion Protein:
  • VF (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains L60S, D96G, and S115G mutations in hADAM10 compared to the NCBI reference sequence NP_001101.1. These mutations were present in the original DNA that was was PCR amplified from human placenta cDNA and are not known to affect plasmid function.