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Addgene

pcDNA3.2-V5-DEST-hSFRP1-GST
(Plasmid #189543)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189543 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.2-V5-DEST
  • Backbone manufacturer
    Customized backbone from Fu lab
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    secreted frizzled related protein 1
  • Alt name
    SFRP1; FRP; FRP1; FrzA; FRP-1; SARP2
  • Species
    H. sapiens (human)
  • Entrez Gene
    SFRP1 (a.k.a. FRP, FRP-1, FRP1, FrzA, SARP2)
  • Tag / Fusion Protein
    • GST (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.2-V5-DEST-hSFRP1-GST was a gift from Haian Fu (Addgene plasmid # 189543 ; http://n2t.net/addgene:189543 ; RRID:Addgene_189543)