pDB2
(Plasmid
#18954)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 18954 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4735
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namephiC31 attB
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Alt nameint
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Alt namephiC31 bacterial attachment site
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)312
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GenBank IDX60952
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (destroyed during cloning)
- 3′ cloning site MluI (destroyed during cloning)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDB2 was a gift from Michele Calos (Addgene plasmid # 18954 ; http://n2t.net/addgene:18954 ; RRID:Addgene_18954) -
For your References section:
PhiC31 integrase mediates integration in cultured synovial cells and enhances gene expression in rabbit joints. Keravala A, Portlock JL, Nash JA, Vitrant DG, Robbins PD, Calos MP. J Gene Med. 2006 Aug . 8(8):1008-17. 10.1002/jgm.928 PubMed 16779871