pMKInt
(Plasmid
#18952)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18952 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMK33
- Backbone size w/o insert (bp) 8500
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Vector typeInsect Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namephiC31 integrase
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SpeciesStreptomyces phage phiC31
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Insert Size (bp)1874
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Entrez Geneint (a.k.a. phiC31p51)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (destroyed during cloning)
- 3′ cloning site EcoRV (destroyed during cloning)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypIJ8600 (Mervyn Bibb, John Innes Institute, Norwich, U.K.)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Map of parent vector is uploaded
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMKInt was a gift from Michele Calos (Addgene plasmid # 18952 ; http://n2t.net/addgene:18952 ; RRID:Addgene_18952) -
For your References section:
Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31. Groth AC, Fish M, Nusse R, Calos MP. Genetics. 2004 Apr . 166(4):1775-82. 10.1534/genetics.166.4.1775 PubMed 15126397
Map uploaded by the depositor.
