pcDNA 3.1(-) VSFP3.1
(Plasmid #18951)

• Depositing Lab: Thomas Knopfel
• Publication: Lundby et al PLoS ONE. 2008 . 3(6):e2514.

Backbone

• Vector backbone: pcDNA3.1(-)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5358
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VSFP3.1
• Alt name: Voltage-Sensitive Fluorescent Protein 3.1
• Species: Ciona intestinalis
• Insert Size (bp): 1445
• GenBank ID: NP_001028998

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

As reported in the accompanying publication (Lundby et al., PLoS ONE, 2008 Jun 25;3(6)), the R217Q mutation has been intently introduced in the S4 domain of Ci-VSP to shift the activation curve of the sensor into the physiological range of neuronal membrane potential.