HA-HIF1alpha-pcDNA3
(Plasmid #18949)

• Depositing Lab: William Kaelin
• Publication: Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5433
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Hypoxia inducible factor 1 alpha
• Alt name: HIF1a
• Species: H. sapiens (human)
• Insert Size (bp): 2505
• Mutation: wt cDNA
• GenBank ID: NM_001530
• Entrez Gene: HIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
• Tag / Fusion Protein:
  • HA-tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH I (not destroyed)
• 3′ cloning site: EcoR I (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Test
• Articles Citing this Plasmid:
  • 66 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry