pDrBB2
(Plasmid #18946)

• Depositing Lab: Michele Calos
• Publication: Groth et al Genetics. 2004 Apr . 166(4):1775-82.

Backbone

• Vector backbone: pHZ-attB
• Backbone size w/o insert (bp): 4622
• Vector type: Insect Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hygromycin gene driven by the copia promoter, phiC31 attB
• Alt name: phiC31 bacterial attachment site
• Species: Escherichia coli
• Insert Size (bp): 3686

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PvuII (destroyed during cloning)
• 3′ cloning site: PflMI (destroyed during cloning)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: hygromycin gene driven by the copia promoter from pMK33 (R. Nusse, Stanford University)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry