pCaryP
(Plasmid #18945)

• Depositing Lab: Michele Calos
• Publication: Groth et al Genetics. 2004 Apr . 166(4):1775-82.

Backbone

• Vector backbone: pCary
• Backbone size w/o insert (bp): 9000
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: phiC31 attP
• Alt name: int
• Alt name: phiC31 phage attachment site
• Species: Streptomyces phage phiC31
• Insert Size (bp): 221

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SmaI (destroyed during cloning)
• 3′ cloning site: SmaI (destroyed during cloning)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: the plasmid pIJ8600 (a gift from Mervyn Bibb, John Innes Institute, Norwich, U.K.)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

attP residues 1123 to 1345
P-element residues 1386 to 1976
P-element residues 4560 to 4966