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Addgene

pUASTB
(Plasmid #18944)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18944 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUAST
  • Backbone size w/o insert (bp) 7804
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    phiC31 attB
  • Alt name
    phiC31 bacterial attachment site
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    279
  • GenBank ID
    X60952

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

AttB residues 8033-228

mini-white residues 229-4384

P Element residues 7556-7962,
4385-4976

Amp residues 5987-6829

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUASTB was a gift from Michele Calos (Addgene plasmid # 18944 ; http://n2t.net/addgene:18944 ; RRID:Addgene_18944)

  • For your References section:

    Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31. Groth AC, Fish M, Nusse R, Calos MP. Genetics. 2004 Apr . 166(4):1775-82. 10.1534/genetics.166.4.1775 PubMed 15126397
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