pET11phiC31poly(A)
(Plasmid #18942)

• Depositing Lab: Michele Calos
• Publication: Groth et al Genetics. 2004 Apr . 166(4):1775-82.

Backbone

• Vector backbone: pET11
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5632
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: phiC31 integrase
• Species: Streptomyces phage phiC31
• Insert Size (bp): 1879
• Entrez Gene: int (a.k.a. phiC31p51)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: the plasmid pIJ8600 from Mervyn Bibb, John Innes Institute, Norwich, U.K.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry