pInt
(Plasmid #18941)

• Depositing Lab: Michele Calos
• Publication: Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 97(11):5995-6000.

Backbone

• Vector backbone: pACYC177
• Backbone size w/o insert (bp): 3016
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: phiC31 integrase
• Species: Streptomyces phage phiC31
• Insert Size (bp): 2182
• Mutation: under lacZ promoter
• Entrez Gene: int (a.k.a. phiC31p51)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PstI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: phiC31 integrase from the plasmid pIJ8600 (Mervyn Bibb, John Innes Institute, Norwich, U.K.)
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry