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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18920 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneEGIP
- Backbone size w/o insert (bp) 10000
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Vector typeMammalian Expression, Lentiviral, Cre/Lox
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsStbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNANOG
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SpeciesH. sapiens (human)
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Insert Size (bp)920
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GenBank IDNM_024865
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Entrez GeneNANOG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer Ef1a (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ef1a_NANOG_Ires_Puro was a gift from Linzhao Cheng (Addgene plasmid # 18920 ; http://n2t.net/addgene:18920 ; RRID:Addgene_18920) -
For your References section:
Improved efficiency and pace of generating induced pluripotent stem cells from human adult and fetal fibroblasts. Mali P, Ye Z, Hommond HH, Yu X, Lin J, Chen G, Zou J, Cheng L. Stem Cells. 2008 Aug . 26(8):1998-2005. 10.1634/stemcells.2008-0346 PubMed 18511599