Skip to main content
Addgene

p70 S6 kinase
(Plasmid #1892)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1892 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    PMT2
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p70 alpha1 S6 kinase
  • Alt name
    p70 S6 kinase
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1800
  • Entrez Gene
    Rps6kb1 (a.k.a. p70 S6K-alpha)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p70 S6 kinase was a gift from Joseph Avruch (Addgene plasmid # 1892 ; http://n2t.net/addgene:1892 ; RRID:Addgene_1892)

  • For your References section:

    Multiple independent inputs are required for activation of the p70 S6 kinase. Weng QP, Andrabi K, Kozlowski MT, Grove JR, Avruch J. Mol Cell Biol 1995 May;15(5):2333-40. PubMed 7739516
Related items:
Commonly requested with: