tetO7_Ef1a_OCT4
(Plasmid
#18918)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18918 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneDuet101
- Backbone size w/o insert (bp) 10000
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Vector typeMammalian Expression, Lentiviral, Cre/Lox
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Selectable markersNone
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsStbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOCT4
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SpeciesH. sapiens (human)
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Insert Size (bp)1100
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GenBank IDNM_002701
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Entrez GenePOU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xba1 (destroyed during cloning)
- 3′ cloning site Cla1 (destroyed during cloning)
- 5′ sequencing primer Ef1a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
tetO7_Ef1a_OCT4 was a gift from Linzhao Cheng (Addgene plasmid # 18918 ; http://n2t.net/addgene:18918 ; RRID:Addgene_18918) -
For your References section:
Improved efficiency and pace of generating induced pluripotent stem cells from human adult and fetal fibroblasts. Mali P, Ye Z, Hommond HH, Yu X, Lin J, Chen G, Zou J, Cheng L. Stem Cells. 2008 Aug . 26(8):1998-2005. 10.1634/stemcells.2008-0346 PubMed 18511599