pYBS187
(Plasmid
#18901)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 18901 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYBS311
- Backbone size w/o insert (bp) 11000
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCdc28
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneCDC28 (a.k.a. YBR160W, CDK1, HSL5, SRM5)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pHybLex_fwd
- 3′ sequencing primer pHybLex-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Also called LexA-Cdc28
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYBS187 was a gift from Elaine Elion (Addgene plasmid # 18901 ; http://n2t.net/addgene:18901 ; RRID:Addgene_18901)