pYBS139
(Plasmid
#18900)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 18900 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJG4-5
- Backbone size w/o insert (bp) 6449
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFAR1
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)6500
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Entrez GeneFAR1 (a.k.a. YJL157C)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pJG4_5_fwd
- 3′ sequencing primer pJG4_5_rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pYBS139 is a derivative of pYBS135 in which the EcoRI site was filled n to create the proper reading frame.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYBS139 was a gift from Elaine Elion (Addgene plasmid # 18900 ; http://n2t.net/addgene:18900 ; RRID:Addgene_18900) -
For your References section:
The MAP kinase Fus3 associates with and phosphorylates the upstream signaling component Ste5. Kranz JE, Satterberg B, Elion EA. Genes Dev. 1994 Feb 1. 8(3):313-27. 10.1101/gad.8.3.313 PubMed 8314085