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pET22b-T5-mad32TAG35TAG
(Plasmid #188999)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188999 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET
  • Backbone size w/o insert (bp) 5608
  • Total vector size (bp) 5809
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    madanin-1 with TAG at 32 and 35 position
  • Insert Size (bp)
    201

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET22b-T5-mad32TAG35TAG was a gift from Han Xiao (Addgene plasmid # 188999 ; http://n2t.net/addgene:188999 ; RRID:Addgene_188999)

  • For your References section:

    Unleashing the potential of noncanonical amino acid biosynthesis to create cells with precision tyrosine sulfation. Chen Y, Jin S, Zhang M, Hu Y, Wu KL, Chung A, Wang S, Tian Z, Wang Y, Wolynes PG, Xiao H. Nat Commun. 2022 Sep 16;13(1):5434. doi: 10.1038/s41467-022-33111-4. 10.1038/s41467-022-33111-4 PubMed 36114189
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