pET22b-T5-mad32TAG
(Plasmid
#188997)
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PurposeExpress madanin-1 with TAG at 32 position
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 188997 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET
- Backbone size w/o insert (bp) 5608
- Total vector size (bp) 5809
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemadanin-1 with TAG at 32 position
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Insert Size (bp)201
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.03.25.485857v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET22b-T5-mad32TAG was a gift from Han Xiao (Addgene plasmid # 188997 ; http://n2t.net/addgene:188997 ; RRID:Addgene_188997) -
For your References section:
Unleashing the potential of noncanonical amino acid biosynthesis to create cells with precision tyrosine sulfation. Chen Y, Jin S, Zhang M, Hu Y, Wu KL, Chung A, Wang S, Tian Z, Wang Y, Wolynes PG, Xiao H. Nat Commun. 2022 Sep 16;13(1):5434. doi: 10.1038/s41467-022-33111-4. 10.1038/s41467-022-33111-4 PubMed 36114189
Map uploaded by the depositor.
