hOCT1-miRFP
(Plasmid #188995)

• Purpose: A mammalian expression plasmid encoding miRFP-tagged human OCT1
• Depositing Lab: Jeremy Baskin
• Publication: Chiu et al JACS Au. 2022 Nov 28;2(12):2703-2713. doi: 10.1021/jacsau.2c00419. eCollection 2022 Dec 26.

Backbone

• Vector backbone: miRFP-N1
• Backbone size w/o insert (bp): 4966
• Total vector size (bp): 6628
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hOCT1
• Alt name: SLC22A1
• Species: H. sapiens (human)
• Insert Size (bp): 1662
• Entrez Gene: SLC22A1 (a.k.a. HOCT1, OCT1, oct1_cds)
• Promoter: CMV
• Tag / Fusion Protein:
  • miRFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-S
• 3′ sequencing primer: miRFP-AS

Resource Information

• Supplemental Documents:
  • hOCT1-miRFP.gb
• A portion of this plasmid was derived from a plasmid made by: Addgene 132034

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry