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pCDH-p18-CIBN
(Plasmid #188990)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188990 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH-CMV-MCS-EF1-Puro
  • Backbone size w/o insert (bp) 7368
  • Total vector size (bp) 8016
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CIBN
  • Alt name
    CIB1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    513
  • Entrez Gene
    CIB1 (a.k.a. AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1)
  • Promoter CMV
  • Tag / Fusion Protein
    • p18 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer Puro-AS (CGACATCACTTTCCCAGTTTAC)
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-p18-CIBN was a gift from Jeremy Baskin (Addgene plasmid # 188990 ; http://n2t.net/addgene:188990 ; RRID:Addgene_188990)

  • For your References section:

    Activity-based directed evolution of a membrane editor in mammalian cells. Tei R, Bagde SR, Fromme JC, Baskin JM. Nat Chem. 2023 May 22. doi: 10.1038/s41557-023-01214-0. 10.1038/s41557-023-01214-0 PubMed 37217787
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