LexA-Fus3
(Plasmid
#18899)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18899 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneLex202pl
- Backbone size w/o insert (bp) 10100
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFUS3
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneFUS3 (a.k.a. YBL016W, DAC2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pHyblex-for (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LexA-Fus3 was a gift from Elaine Elion (Addgene plasmid # 18899 ; http://n2t.net/addgene:18899 ; RRID:Addgene_18899) -
For your References section:
FUS3 encodes a cdc2+/CDC28-related kinase required for the transition from mitosis into conjugation. Elion EA, Grisafi PL, Fink GR. Cell. 1990 Feb 23. 60(4):649-64. 10.1016/0092-8674(90)90668-5 PubMed 2406028