pCDH-CRY2-mCh-superPLDx30
(Plasmid
#188988)
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PurposeA lentiviral plasmid encoding CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 188988 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDH-CMV-MCS-EF1-Puro
- Backbone size w/o insert (bp) 7368
- Total vector size (bp) 11167
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePLD
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Alt namePhospholipase D
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SpeciesStreptomyces PMF
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Insert Size (bp)1527
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MutationK109R, P245A, G328S, G381V, G429D
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Tags
/ Fusion Proteins
- CRY2 (N terminal on insert)
- mCherry (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDH-CRY2-mCh-superPLDx30 was a gift from Jeremy Baskin (Addgene plasmid # 188988 ; http://n2t.net/addgene:188988 ; RRID:Addgene_188988) -
For your References section:
Activity-based directed evolution of a membrane editor in mammalian cells. Tei R, Bagde SR, Fromme JC, Baskin JM. Nat Chem. 2023 May 22. doi: 10.1038/s41557-023-01214-0. 10.1038/s41557-023-01214-0 PubMed 37217787
Map uploaded by the depositor.
