pCDH-CRY2-mCh-superPLDx30
(Plasmid #188988)

• Purpose: A lentiviral plasmid encoding CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type)
• Depositing Lab: Jeremy Baskin
• Publication: Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s41557-023-01214-0.

Backbone

• Vector backbone: pCDH-CMV-MCS-EF1-Puro
• Backbone size w/o insert (bp): 7368
• Total vector size (bp): 11167
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PLD
• Alt name: Phospholipase D
• Species: Streptomyces PMF
• Insert Size (bp): 1527
• Mutation: K109R, P245A, G328S, G381V, G429D
• Tags / Fusion Proteins:
  • CRY2 (N terminal on insert)
  • mCherry (N terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry