pEvol-NnSULT1C1-cysDNCQ
(Plasmid
#188983)
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Purposebiosynthesize sulfotyrosine in E. coli.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 188983 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEvol
- Backbone size w/o insert (bp) 3325
- Total vector size (bp) 8394
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameNnSULT1C1
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Insert Size (bp)924
Gene/Insert 2
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Gene/Insert namecysD
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Insert Size (bp)908
Gene/Insert 3
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Gene/Insert namecysN
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Insert Size (bp)1425
Gene/Insert 4
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Gene/Insert namecysC
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Insert Size (bp)606
Gene/Insert 5
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Gene/Insert namecysQ
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Insert Size (bp)741
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.03.25.485857v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEvol-NnSULT1C1-cysDNCQ was a gift from Han Xiao (Addgene plasmid # 188983 ; http://n2t.net/addgene:188983 ; RRID:Addgene_188983) -
For your References section:
Unleashing the potential of noncanonical amino acid biosynthesis to create cells with precision tyrosine sulfation. Chen Y, Jin S, Zhang M, Hu Y, Wu KL, Chung A, Wang S, Tian Z, Wang Y, Wolynes PG, Xiao H. Nat Commun. 2022 Sep 16;13(1):5434. doi: 10.1038/s41467-022-33111-4. 10.1038/s41467-022-33111-4 PubMed 36114189