pET3a-Ub-K63R
(Plasmid #18898)

• Depositing Lab: Cecile Pickart
• Publication: Raasi et al Methods Mol Biol. 2005 . 301():47-55.

Backbone

• Vector backbone: pET3a
• Backbone size w/o insert (bp): 4600
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: BL21pJY2
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Ubiquitin-K63R
• Alt name: ubc
• Species: H. sapiens (human)
• Mutation: Lysine 63 is replaced with a Argnine residue
• Entrez Gene: UBC (a.k.a. HMG20)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Plasmid is constructed by R. Raguraj.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Tsui C, Raguraj A, Pickart CM.
J Biol Chem. 2005 May 20;280(20):19829-35. Epub 2005 Mar 16.