pREDusk-AmpR-MCS
(Plasmid
#188974)
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Purpose(Empty Backbone) For red light-repressed bacterial expression of gene of interest (AmpR)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 188974 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
- 3′ sequencing primer T7term (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pREDusk-AmpR-MCS was a gift from Heikki Takala (Addgene plasmid # 188974 ; http://n2t.net/addgene:188974 ; RRID:Addgene_188974) -
For your References section:
Optogenetic Control of Bacterial Expression by Red Light. Multamaki E, Garcia de Fuentes A, Sieryi O, Bykov A, Gerken U, Ranzani AT, Kohler J, Meglinski I, Moglich A, Takala H. ACS Synth Biol. 2022 Aug 23. doi: 10.1021/acssynbio.2c00259. 10.1021/acssynbio.2c00259 PubMed 35998606
