pET3a-Ub-K48C
(Plasmid #18897)

• Depositing Lab: Cecile Pickart
• Publication: Raasi et al Methods Mol Biol. 2005 . 301():47-55.

Backbone

• Vector backbone: pET3a
• Backbone size w/o insert (bp): 4600
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: BL21pJY2
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Ubiquitin-K48C
• Alt name: UBC
• Species: H. sapiens (human)
• Mutation: Lysine 48 is replaced with a Cysteine residue
• Entrez Gene: UBC (a.k.a. HMG20)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Beal R. has constructed this plasmid.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Beal RE, Toscano-Cantaffa D, Young P, Rechsteiner M, Pickart CM. Biochemistry. 1998 Mar 3;37(9):2925-34