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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18895 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneN/A
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUbiquitin C-terminal hydrolase I
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Alt nameYUH1
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneYUH1 (a.k.a. YJR099W)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPlasmid is obtained from Robert E. Cohen.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Johnston SC, Riddle SM, Cohen RE, Hill CP. EMBO J. 1999 Jul 15;18(14):3877-87
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET3a-Yuh1 was a gift from Cecile Pickart (Addgene plasmid # 18895 ; http://n2t.net/addgene:18895 ; RRID:Addgene_18895) -
For your References section:
Ubiquitin chain synthesis. Raasi S, Pickart CM. Methods Mol Biol. 2005 . 301():47-55. 10.1385/1-59259-895-1:047 PubMed 15917625