Tol2pA2-drl:APEX2-mCherry
(Plasmid #188945)

• Purpose: Plasmid for Tol2 transgenesis expressing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry, driven by the draculin promoter
• Depositing Lab: Owen Tamplin
• Publication: Agarwala et al Elife. 2022 Aug 9;11. pii: 64835. doi: 10.7554/eLife.64835.

Backbone

• Vector backbone: 394.pDestTol2pA2
• Backbone manufacturer: Tol2Kit
• Backbone size w/o insert (bp): 5883
• Total vector size (bp): 13750
• Vector type: Tol2 destination vector (attR4-R3)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: drl:mito-APEX2_p2A_APEX2-H2B_p2A_mCherry_polyA
• Species: Synthetic
• Insert Size (bp): 9571

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13R
• 3′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Tol2pA2 backbone from Tol2Kit (Kristen Kwan), draculin (drl) promoter from Christian Mosimann (pCM293)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.11.12.380790v1 for bioRxiv preprint.